67.5 Active B12

67.5.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 250.7924480 1 <0.0001 ***
Study 3.2136720 1 0.07 ns
f39_gdm_new 0.5754347 1 0.4 ns
BMI 3.3368238 1 0.07 ns
Ethnicity_cauc 105.4219556 1 <0.0001 ***
f10c_any_cig_1st_vst 49.1590682 1 <0.0001 ***
GA_samp 164.6323095 1 <0.0001 ***
Study:f39_gdm_new 1.8652579 1 0.2 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 150.8759535 1 <0.0001 ***
Study 7.3986291 1 0.007 **
f39_gdm_new 0.4357432 1 0.5 ns
BMI 236.2094019 1 <0.0001 ***
Ethnicity_cauc 5.5165373 1 0.02 *
f10c_any_cig_1st_vst 0.9176455 1 0.3 ns
GA_samp 2601.9722143 1 <0.0001 ***
Study:f39_gdm_new 1.4845286 1 0.2 ns
GH2
Chisq Df Pr(>Chisq)
(Intercept) 251.096669 1 <0.0001 ***
Study 1.107622 1 0.3 ns
f39_gdm_new 1.386104 1 0.2 ns
BMI 87.622496 1 <0.0001 ***
Ethnicity_cauc 3.246832 1 0.07 ns
f10c_any_cig_1st_vst 1.039153 1 0.3 ns
GA_samp 1032.266869 1 <0.0001 ***
Study:f39_gdm_new 3.376951 1 0.07 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 21.326 21.326 <0.0001 ***
3 f39_gdm_new 1 Inf 33.726 33.726 <0.0001 ***
4 BMI 1 Inf 330.722 330.722 <0.0001 ***
5 Ethnicity_cauc 1 Inf 25.046 25.046 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 3.158 3.158 0.08 ns
7 GA_samp 1 Inf 2.506 2.506 0.1 ns
2 Study:f39_gdm_new 1 Inf 4.089 4.089 0.04 *
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 36.051 36.051 <0.0001 ***
3 f39_gdm_new 1 Inf 32.027 32.027 <0.0001 ***
4 BMI 1 Inf 316.807 316.807 <0.0001 ***
5 Ethnicity_cauc 1 Inf 24.882 24.882 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 2.618 2.618 0.1 ns
7 GA_samp 1 Inf 1.602 1.602 0.2 ns
2 Study:f39_gdm_new 1 Inf 4.361 4.361 0.04 *
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 53.182 53.182 <0.0001 ***
3 f39_gdm_new 1 Inf 4.345 4.345 0.04 *
4 BMI 1 Inf 11.449 11.449 0.0007 ***
5 Ethnicity_cauc 1 Inf 0.718 0.718 0.4 ns
6 f10c_any_cig_1st_vst 1 Inf 0.028 0.028 0.9 ns
7 GA_samp 1 Inf 0.278 0.278 0.6 ns
2 Study:f39_gdm_new 1 Inf 1.558 1.558 0.2 ns

67.5.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.77 (2.27, 3.26) <0.0001
Active.B12 0.00 (-0.00, 0.00) 0.10
f39_gdm_newGDM -0.25 (-0.70, 0.20) 0.27
StudySTOP -0.02 (-0.26, 0.22) 0.88
GA_samp 0.13 (0.10, 0.16) <0.0001
Active.B12:f39_gdm_newGDM 0.00 (-0.00, 0.01) 0.90
f39_gdm_newGDM:StudySTOP 0.22 (-0.09, 0.54) 0.15
Active.B12:StudySTOP -0.00 (-0.01, 0.00) 0.24
HPL
Var Effect P
(Intercept) 0.68 (0.42, 0.94) <0.0001
Active.B12 -0.00 (-0.00, 0.00) 0.70
f39_gdm_newGDM -0.29 (-0.52, -0.06) 0.01
StudySTOP 0.09 (-0.04, 0.22) 0.19
GA_samp 0.29 (0.27, 0.30) <0.0001
Active.B12:f39_gdm_newGDM 0.00 (0.00, 0.01) 0.05
f39_gdm_newGDM:StudySTOP 0.11 (-0.04, 0.27) 0.14
Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.72
GH2
Var Effect P
(Intercept) -2.77 (-3.12, -2.42) <0.0001
Active.B12 0.00 (-0.00, 0.00) 0.24
f39_gdm_newGDM -0.33 (-0.66, -0.01) 0.04
StudySTOP 0.12 (-0.06, 0.30) 0.19
GA_samp 0.27 (0.25, 0.29) <0.0001
Active.B12:f39_gdm_newGDM 0.00 (-0.00, 0.01) 0.28
f39_gdm_newGDM:StudySTOP 0.20 (-0.01, 0.42) 0.07
Active.B12:StudySTOP -0.00 (-0.00, -0.00) 0.05
Insulin
Var Effect P
(Intercept) 2.48 (1.84, 3.11) <0.0001
Active.B12 0.00 (-0.00, 0.01) 0.13
f39_gdm_newGDM 1.74 (0.69, 2.80) 0.001
StudySTOP -0.11 (-0.46, 0.23) 0.52
GA_samp 0.01 (-0.03, 0.04) 0.76
Active.B12:f39_gdm_newGDM -0.01 (-0.03, 0.00) 0.11
f39_gdm_newGDM:StudySTOP -1.03 (-2.28, 0.22) 0.11
Active.B12:StudySTOP -0.00 (-0.01, 0.00) 0.18
Active.B12:f39_gdm_newGDM:StudySTOP 0.01 (-0.01, 0.03) 0.26
HOMA.IR
Var Effect P
(Intercept) 1.14 (0.46, 1.82) 0.0010
Active.B12 0.00 (-0.00, 0.01) 0.09
f39_gdm_newGDM 1.95 (0.73, 3.17) 0.002
StudySTOP -0.23 (-0.60, 0.14) 0.23
GA_samp -0.00 (-0.04, 0.04) 0.91
Active.B12:f39_gdm_newGDM -0.01 (-0.03, 0.00) 0.09
f39_gdm_newGDM:StudySTOP -1.21 (-2.63, 0.20) 0.09
Active.B12:StudySTOP -0.00 (-0.01, 0.00) 0.11
Active.B12:f39_gdm_newGDM:StudySTOP 0.01 (-0.01, 0.03) 0.22
GLUC3_mmol.L
Var Effect P
(Intercept) 1.78 (1.66, 1.91) <0.0001
Active.B12 0.00 (-0.00, 0.00) 0.69
f39_gdm_newGDM 0.28 (-0.02, 0.59) 0.06
StudySTOP -0.18 (-0.25, -0.11) <0.0001
GA_samp -0.01 (-0.01, 0.00) 0.12
Active.B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.06
f39_gdm_newGDM:StudySTOP -0.13 (-0.30, 0.04) 0.14
Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.53

67.5.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Active B12 concentration (pM) and may be misleading when interaction term is involved.

Prolactin
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 105.47360 8.797897 100.46567 89.38747 124.4546
STOP non-GDM 91.28296 7.311908 85.90561 77.84542 107.0401
SCOPE GDM 83.82405 13.286430 654.99319 61.40450 114.4293
STOP GDM 90.81424 9.510369 227.38215 73.88160 111.6276
HPL
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 93.33578 2.821670 211.1402 87.93602 99.06711
STOP non-GDM 103.94928 2.957134 170.7303 98.27290 109.95354
SCOPE GDM 82.17595 5.845062 532.8276 71.45985 94.49903
STOP GDM 102.60359 4.718066 563.5327 93.74263 112.30213
GH2
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 2.447513 0.0848546 355.6125 2.286195 2.620214
STOP non-GDM 2.358520 0.0749692 313.8559 2.215532 2.510736
SCOPE GDM 1.994865 0.1989180 729.4797 1.640193 2.426231
STOP GDM 2.359288 0.1347494 640.0163 2.108983 2.639302
Insulin
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 15.43633 0.9590294 Inf 13.666600 17.43522
STOP non-GDM 11.05675 0.5725429 Inf 9.989654 12.23784
SCOPE GDM 40.65369 7.6148581 Inf 28.161842 58.68659
STOP GDM 19.40057 2.1825681 Inf 15.561599 24.18660
HOMA.IR
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 3.796055 0.2542084 Inf 3.329128 4.328470
STOP non-GDM 2.287413 0.1259047 Inf 2.053489 2.547984
SCOPE GDM 10.673449 2.3378803 Inf 6.948005 16.396435
STOP GDM 4.133515 0.4987094 Inf 3.263036 5.236211
GLUC3_mmol.L
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 5.504089 0.0649246 Inf 5.378299 5.632821
STOP non-GDM 4.721598 0.0523972 Inf 4.620010 4.825419
SCOPE GDM 6.412928 0.5706738 Inf 5.386536 7.634895
STOP GDM 4.828312 0.1019511 Inf 4.632570 5.032325

67.5.4 Comparisons

67.5.4.1 Within Groups

Effect for every 10 unit increase in Active B12 concentration (pM).
Hormone Contrast Study f39_gdm_new Ratio of Geometric means (95% CI) P-value
Prolactin Active.B1271 / Active.B1261 SCOPE non-GDM 1.020 (0.996, 1.045) 0.10
Prolactin Active.B1271 / Active.B1261 STOP non-GDM 1.001 (0.979, 1.024) 0.9
Prolactin Active.B1271 / Active.B1261 SCOPE GDM 1.023 (0.973, 1.076) 0.4
Prolactin Active.B1271 / Active.B1261 STOP GDM 1.004 (0.960, 1.050) 0.9
HPL Active.B1271 / Active.B1261 SCOPE non-GDM 0.997 (0.985, 1.010) 0.7
HPL Active.B1271 / Active.B1261 STOP non-GDM 1.001 (0.989, 1.013) 0.9
HPL Active.B1271 / Active.B1261 SCOPE GDM 1.023 (0.996, 1.050) 0.09
HPL Active.B1271 / Active.B1261 STOP GDM 1.026 (1.002, 1.051) 0.03
GH2 Active.B1271 / Active.B1261 SCOPE non-GDM 1.011 (0.993, 1.028) 0.2
GH2 Active.B1271 / Active.B1261 STOP non-GDM 0.987 (0.971, 1.003) 0.1
GH2 Active.B1271 / Active.B1261 SCOPE GDM 1.031 (0.993, 1.070) 0.1
GH2 Active.B1271 / Active.B1261 STOP GDM 1.007 (0.974, 1.040) 0.7
Insulin Active.B1271 / Active.B1261 SCOPE non-GDM 1.028 (0.992, 1.066) 0.1
Insulin Active.B1271 / Active.B1261 STOP non-GDM 0.995 (0.963, 1.027) 0.7
Insulin Active.B1271 / Active.B1261 SCOPE GDM 0.914 (0.796, 1.049) 0.2
Insulin Active.B1271 / Active.B1261 STOP GDM 0.972 (0.900, 1.049) 0.5
HOMA.IR Active.B1271 / Active.B1261 SCOPE non-GDM 1.035 (0.995, 1.076) 0.09
HOMA.IR Active.B1271 / Active.B1261 STOP non-GDM 0.992 (0.960, 1.026) 0.6
HOMA.IR Active.B1271 / Active.B1261 SCOPE GDM 0.900 (0.770, 1.053) 0.2
HOMA.IR Active.B1271 / Active.B1261 STOP GDM 0.970 (0.892, 1.056) 0.5
GLUC3_mmol.L Active.B1271 / Active.B1261 SCOPE non-GDM 1.001 (0.995, 1.008) 0.7
GLUC3_mmol.L Active.B1271 / Active.B1261 STOP non-GDM 1.005 (0.997, 1.013) 0.2
GLUC3_mmol.L Active.B1271 / Active.B1261 SCOPE GDM 0.982 (0.959, 1.005) 0.1
GLUC3_mmol.L Active.B1271 / Active.B1261 STOP GDM 0.985 (0.968, 1.003) 0.10

67.5.4.2 Between Groups

Below are comparisons of the differences in the change in Active B12 concentration (pM) between groups. I.e. whether the change in Active B12 concentration (pM) are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.962618 0.0313047 1074.736 0.9031115 1.026045 0.242 ns
STOP/SCOPE (non-GDM) 0.981131 0.0159534 1074.736 0.9503218 1.012939 0.242 ns
STOP/SCOPE (GDM) 0.981131 0.0159534 1074.736 0.9503218 1.012939 0.242 ns
GDM/non-GDM (SCOPE) 1.002941 0.0244402 1057.071 0.9561131 1.052063 0.904 ns
GDM/non-GDM (STOP) 1.002941 0.0244402 1057.071 0.9561131 1.052063 0.904 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.006290 0.0175121 1052.3229 0.9725073 1.041246 0.719 ns
STOP/SCOPE (non-GDM) 1.003140 0.0087287 1052.3229 0.9861578 1.020415 0.719 ns
STOP/SCOPE (GDM) 1.003140 0.0087287 1052.3229 0.9861578 1.020415 0.719 ns
GDM/non-GDM (SCOPE) 1.025411 0.0131357 958.8608 0.9999541 1.051515 0.050 ns
GDM/non-GDM (STOP) 1.025411 0.0131357 958.8608 0.9999541 1.051515 0.050 ns
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9539858 0.0227571 1107.714 0.9103627 0.9996992 0.049 *
STOP/SCOPE (non-GDM) 0.9767220 0.0116497 1107.714 0.9541293 0.9998496 0.049 *
STOP/SCOPE (GDM) 0.9767220 0.0116497 1107.714 0.9541293 0.9998496 0.049 *
GDM/non-GDM (SCOPE) 1.0197965 0.0183647 1081.377 0.9843912 1.0564752 0.277 ns
GDM/non-GDM (STOP) 1.0197965 0.0183647 1081.377 0.9843912 1.0564752 0.277 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0284138 0.0865689 Inf 0.8719995 1.212885 0.739 ns
STOP/SCOPE (non-GDM) 0.9673981 0.0237372 Inf 0.9219751 1.015059 0.177 ns
STOP/SCOPE (GDM) 1.0630720 0.0857006 Inf 0.9076998 1.245040 0.448 ns
GDM/non-GDM (SCOPE) 0.8892404 0.0647259 Inf 0.7710136 1.025596 0.107 ns
GDM/non-GDM (STOP) 0.9771846 0.0414479 Inf 0.8992333 1.061893 0.586 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0334534 0.0973941 Inf 0.8591568 1.243109 0.727 ns
STOP/SCOPE (non-GDM) 0.9588863 0.0250776 Inf 0.9109735 1.009319 0.108 ns
STOP/SCOPE (GDM) 1.0777643 0.0976936 Inf 0.9023331 1.287303 0.409 ns
GDM/non-GDM (SCOPE) 0.8701767 0.0714202 Inf 0.7408744 1.022046 0.090 ns
GDM/non-GDM (STOP) 0.9780569 0.0454341 Inf 0.8929412 1.071286 0.633 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.007084 0.0112788 Inf 0.9852193 1.029435 0.528 ns
STOP/SCOPE (non-GDM) 1.003536 0.0056195 Inf 0.9925821 1.014611 0.528 ns
STOP/SCOPE (GDM) 1.003536 0.0056195 Inf 0.9925821 1.014611 0.528 ns
GDM/non-GDM (SCOPE) 0.980328 0.0103639 Inf 0.9602242 1.000853 0.060 ns
GDM/non-GDM (STOP) 0.980328 0.0103639 Inf 0.9602242 1.000853 0.060 ns

67.5.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

67.5.6 Plots