67.5 Active B12
67.5.1 Global tests
Type III ANOVA on main and interaction effects, and LM model summaries.
Prolactin| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 250.7924480 | 1 | <0.0001 | *** |
| Study | 3.2136720 | 1 | 0.07 | ns |
| f39_gdm_new | 0.5754347 | 1 | 0.4 | ns |
| BMI | 3.3368238 | 1 | 0.07 | ns |
| Ethnicity_cauc | 105.4219556 | 1 | <0.0001 | *** |
| f10c_any_cig_1st_vst | 49.1590682 | 1 | <0.0001 | *** |
| GA_samp | 164.6323095 | 1 | <0.0001 | *** |
| Study:f39_gdm_new | 1.8652579 | 1 | 0.2 | ns |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 150.8759535 | 1 | <0.0001 | *** |
| Study | 7.3986291 | 1 | 0.007 | ** |
| f39_gdm_new | 0.4357432 | 1 | 0.5 | ns |
| BMI | 236.2094019 | 1 | <0.0001 | *** |
| Ethnicity_cauc | 5.5165373 | 1 | 0.02 | * |
| f10c_any_cig_1st_vst | 0.9176455 | 1 | 0.3 | ns |
| GA_samp | 2601.9722143 | 1 | <0.0001 | *** |
| Study:f39_gdm_new | 1.4845286 | 1 | 0.2 | ns |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 251.096669 | 1 | <0.0001 | *** |
| Study | 1.107622 | 1 | 0.3 | ns |
| f39_gdm_new | 1.386104 | 1 | 0.2 | ns |
| BMI | 87.622496 | 1 | <0.0001 | *** |
| Ethnicity_cauc | 3.246832 | 1 | 0.07 | ns |
| f10c_any_cig_1st_vst | 1.039153 | 1 | 0.3 | ns |
| GA_samp | 1032.266869 | 1 | <0.0001 | *** |
| Study:f39_gdm_new | 3.376951 | 1 | 0.07 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Study | 1 | Inf | 21.326 | 21.326 | <0.0001 | *** |
| 3 | f39_gdm_new | 1 | Inf | 33.726 | 33.726 | <0.0001 | *** |
| 4 | BMI | 1 | Inf | 330.722 | 330.722 | <0.0001 | *** |
| 5 | Ethnicity_cauc | 1 | Inf | 25.046 | 25.046 | <0.0001 | *** |
| 6 | f10c_any_cig_1st_vst | 1 | Inf | 3.158 | 3.158 | 0.08 | ns |
| 7 | GA_samp | 1 | Inf | 2.506 | 2.506 | 0.1 | ns |
| 2 | Study:f39_gdm_new | 1 | Inf | 4.089 | 4.089 | 0.04 | * |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Study | 1 | Inf | 36.051 | 36.051 | <0.0001 | *** |
| 3 | f39_gdm_new | 1 | Inf | 32.027 | 32.027 | <0.0001 | *** |
| 4 | BMI | 1 | Inf | 316.807 | 316.807 | <0.0001 | *** |
| 5 | Ethnicity_cauc | 1 | Inf | 24.882 | 24.882 | <0.0001 | *** |
| 6 | f10c_any_cig_1st_vst | 1 | Inf | 2.618 | 2.618 | 0.1 | ns |
| 7 | GA_samp | 1 | Inf | 1.602 | 1.602 | 0.2 | ns |
| 2 | Study:f39_gdm_new | 1 | Inf | 4.361 | 4.361 | 0.04 | * |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Study | 1 | Inf | 53.182 | 53.182 | <0.0001 | *** |
| 3 | f39_gdm_new | 1 | Inf | 4.345 | 4.345 | 0.04 | * |
| 4 | BMI | 1 | Inf | 11.449 | 11.449 | 0.0007 | *** |
| 5 | Ethnicity_cauc | 1 | Inf | 0.718 | 0.718 | 0.4 | ns |
| 6 | f10c_any_cig_1st_vst | 1 | Inf | 0.028 | 0.028 | 0.9 | ns |
| 7 | GA_samp | 1 | Inf | 0.278 | 0.278 | 0.6 | ns |
| 2 | Study:f39_gdm_new | 1 | Inf | 1.558 | 1.558 | 0.2 | ns |
67.5.2 Model summary
Ratio of Geometric means (95% CI)
Prolactin| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.77 (2.27, 3.26) | <0.0001 |
| Active.B12 | 0.00 (-0.00, 0.00) | 0.10 |
| f39_gdm_newGDM | -0.25 (-0.70, 0.20) | 0.27 |
| StudySTOP | -0.02 (-0.26, 0.22) | 0.88 |
| GA_samp | 0.13 (0.10, 0.16) | <0.0001 |
| Active.B12:f39_gdm_newGDM | 0.00 (-0.00, 0.01) | 0.90 |
| f39_gdm_newGDM:StudySTOP | 0.22 (-0.09, 0.54) | 0.15 |
| Active.B12:StudySTOP | -0.00 (-0.01, 0.00) | 0.24 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 0.68 (0.42, 0.94) | <0.0001 |
| Active.B12 | -0.00 (-0.00, 0.00) | 0.70 |
| f39_gdm_newGDM | -0.29 (-0.52, -0.06) | 0.01 |
| StudySTOP | 0.09 (-0.04, 0.22) | 0.19 |
| GA_samp | 0.29 (0.27, 0.30) | <0.0001 |
| Active.B12:f39_gdm_newGDM | 0.00 (0.00, 0.01) | 0.05 |
| f39_gdm_newGDM:StudySTOP | 0.11 (-0.04, 0.27) | 0.14 |
| Active.B12:StudySTOP | 0.00 (-0.00, 0.00) | 0.72 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | -2.77 (-3.12, -2.42) | <0.0001 |
| Active.B12 | 0.00 (-0.00, 0.00) | 0.24 |
| f39_gdm_newGDM | -0.33 (-0.66, -0.01) | 0.04 |
| StudySTOP | 0.12 (-0.06, 0.30) | 0.19 |
| GA_samp | 0.27 (0.25, 0.29) | <0.0001 |
| Active.B12:f39_gdm_newGDM | 0.00 (-0.00, 0.01) | 0.28 |
| f39_gdm_newGDM:StudySTOP | 0.20 (-0.01, 0.42) | 0.07 |
| Active.B12:StudySTOP | -0.00 (-0.00, -0.00) | 0.05 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.48 (1.84, 3.11) | <0.0001 |
| Active.B12 | 0.00 (-0.00, 0.01) | 0.13 |
| f39_gdm_newGDM | 1.74 (0.69, 2.80) | 0.001 |
| StudySTOP | -0.11 (-0.46, 0.23) | 0.52 |
| GA_samp | 0.01 (-0.03, 0.04) | 0.76 |
| Active.B12:f39_gdm_newGDM | -0.01 (-0.03, 0.00) | 0.11 |
| f39_gdm_newGDM:StudySTOP | -1.03 (-2.28, 0.22) | 0.11 |
| Active.B12:StudySTOP | -0.00 (-0.01, 0.00) | 0.18 |
| Active.B12:f39_gdm_newGDM:StudySTOP | 0.01 (-0.01, 0.03) | 0.26 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.14 (0.46, 1.82) | 0.0010 |
| Active.B12 | 0.00 (-0.00, 0.01) | 0.09 |
| f39_gdm_newGDM | 1.95 (0.73, 3.17) | 0.002 |
| StudySTOP | -0.23 (-0.60, 0.14) | 0.23 |
| GA_samp | -0.00 (-0.04, 0.04) | 0.91 |
| Active.B12:f39_gdm_newGDM | -0.01 (-0.03, 0.00) | 0.09 |
| f39_gdm_newGDM:StudySTOP | -1.21 (-2.63, 0.20) | 0.09 |
| Active.B12:StudySTOP | -0.00 (-0.01, 0.00) | 0.11 |
| Active.B12:f39_gdm_newGDM:StudySTOP | 0.01 (-0.01, 0.03) | 0.22 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.78 (1.66, 1.91) | <0.0001 |
| Active.B12 | 0.00 (-0.00, 0.00) | 0.69 |
| f39_gdm_newGDM | 0.28 (-0.02, 0.59) | 0.06 |
| StudySTOP | -0.18 (-0.25, -0.11) | <0.0001 |
| GA_samp | -0.01 (-0.01, 0.00) | 0.12 |
| Active.B12:f39_gdm_newGDM | -0.00 (-0.00, 0.00) | 0.06 |
| f39_gdm_newGDM:StudySTOP | -0.13 (-0.30, 0.04) | 0.14 |
| Active.B12:StudySTOP | 0.00 (-0.00, 0.00) | 0.53 |
67.5.3 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of Active B12 concentration (pM) and may be misleading when interaction term is involved.
| Study | f39_gdm_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 105.47360 | 8.797897 | 100.46567 | 89.38747 | 124.4546 |
| STOP | non-GDM | 91.28296 | 7.311908 | 85.90561 | 77.84542 | 107.0401 |
| SCOPE | GDM | 83.82405 | 13.286430 | 654.99319 | 61.40450 | 114.4293 |
| STOP | GDM | 90.81424 | 9.510369 | 227.38215 | 73.88160 | 111.6276 |
| Study | f39_gdm_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 93.33578 | 2.821670 | 211.1402 | 87.93602 | 99.06711 |
| STOP | non-GDM | 103.94928 | 2.957134 | 170.7303 | 98.27290 | 109.95354 |
| SCOPE | GDM | 82.17595 | 5.845062 | 532.8276 | 71.45985 | 94.49903 |
| STOP | GDM | 102.60359 | 4.718066 | 563.5327 | 93.74263 | 112.30213 |
| Study | f39_gdm_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 2.447513 | 0.0848546 | 355.6125 | 2.286195 | 2.620214 |
| STOP | non-GDM | 2.358520 | 0.0749692 | 313.8559 | 2.215532 | 2.510736 |
| SCOPE | GDM | 1.994865 | 0.1989180 | 729.4797 | 1.640193 | 2.426231 |
| STOP | GDM | 2.359288 | 0.1347494 | 640.0163 | 2.108983 | 2.639302 |
| Study | f39_gdm_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 15.43633 | 0.9590294 | Inf | 13.666600 | 17.43522 |
| STOP | non-GDM | 11.05675 | 0.5725429 | Inf | 9.989654 | 12.23784 |
| SCOPE | GDM | 40.65369 | 7.6148581 | Inf | 28.161842 | 58.68659 |
| STOP | GDM | 19.40057 | 2.1825681 | Inf | 15.561599 | 24.18660 |
| Study | f39_gdm_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 3.796055 | 0.2542084 | Inf | 3.329128 | 4.328470 |
| STOP | non-GDM | 2.287413 | 0.1259047 | Inf | 2.053489 | 2.547984 |
| SCOPE | GDM | 10.673449 | 2.3378803 | Inf | 6.948005 | 16.396435 |
| STOP | GDM | 4.133515 | 0.4987094 | Inf | 3.263036 | 5.236211 |
| Study | f39_gdm_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 5.504089 | 0.0649246 | Inf | 5.378299 | 5.632821 |
| STOP | non-GDM | 4.721598 | 0.0523972 | Inf | 4.620010 | 4.825419 |
| SCOPE | GDM | 6.412928 | 0.5706738 | Inf | 5.386536 | 7.634895 |
| STOP | GDM | 4.828312 | 0.1019511 | Inf | 4.632570 | 5.032325 |
67.5.4 Comparisons
67.5.4.1 Within Groups
Effect for every 10 unit increase in Active B12 concentration (pM).| Hormone | Contrast | Study | f39_gdm_new | Ratio of Geometric means (95% CI) | P-value |
|---|---|---|---|---|---|
| Prolactin | Active.B1271 / Active.B1261 | SCOPE | non-GDM | 1.020 (0.996, 1.045) | 0.10 |
| Prolactin | Active.B1271 / Active.B1261 | STOP | non-GDM | 1.001 (0.979, 1.024) | 0.9 |
| Prolactin | Active.B1271 / Active.B1261 | SCOPE | GDM | 1.023 (0.973, 1.076) | 0.4 |
| Prolactin | Active.B1271 / Active.B1261 | STOP | GDM | 1.004 (0.960, 1.050) | 0.9 |
| HPL | Active.B1271 / Active.B1261 | SCOPE | non-GDM | 0.997 (0.985, 1.010) | 0.7 |
| HPL | Active.B1271 / Active.B1261 | STOP | non-GDM | 1.001 (0.989, 1.013) | 0.9 |
| HPL | Active.B1271 / Active.B1261 | SCOPE | GDM | 1.023 (0.996, 1.050) | 0.09 |
| HPL | Active.B1271 / Active.B1261 | STOP | GDM | 1.026 (1.002, 1.051) | 0.03 |
| GH2 | Active.B1271 / Active.B1261 | SCOPE | non-GDM | 1.011 (0.993, 1.028) | 0.2 |
| GH2 | Active.B1271 / Active.B1261 | STOP | non-GDM | 0.987 (0.971, 1.003) | 0.1 |
| GH2 | Active.B1271 / Active.B1261 | SCOPE | GDM | 1.031 (0.993, 1.070) | 0.1 |
| GH2 | Active.B1271 / Active.B1261 | STOP | GDM | 1.007 (0.974, 1.040) | 0.7 |
| Insulin | Active.B1271 / Active.B1261 | SCOPE | non-GDM | 1.028 (0.992, 1.066) | 0.1 |
| Insulin | Active.B1271 / Active.B1261 | STOP | non-GDM | 0.995 (0.963, 1.027) | 0.7 |
| Insulin | Active.B1271 / Active.B1261 | SCOPE | GDM | 0.914 (0.796, 1.049) | 0.2 |
| Insulin | Active.B1271 / Active.B1261 | STOP | GDM | 0.972 (0.900, 1.049) | 0.5 |
| HOMA.IR | Active.B1271 / Active.B1261 | SCOPE | non-GDM | 1.035 (0.995, 1.076) | 0.09 |
| HOMA.IR | Active.B1271 / Active.B1261 | STOP | non-GDM | 0.992 (0.960, 1.026) | 0.6 |
| HOMA.IR | Active.B1271 / Active.B1261 | SCOPE | GDM | 0.900 (0.770, 1.053) | 0.2 |
| HOMA.IR | Active.B1271 / Active.B1261 | STOP | GDM | 0.970 (0.892, 1.056) | 0.5 |
| GLUC3_mmol.L | Active.B1271 / Active.B1261 | SCOPE | non-GDM | 1.001 (0.995, 1.008) | 0.7 |
| GLUC3_mmol.L | Active.B1271 / Active.B1261 | STOP | non-GDM | 1.005 (0.997, 1.013) | 0.2 |
| GLUC3_mmol.L | Active.B1271 / Active.B1261 | SCOPE | GDM | 0.982 (0.959, 1.005) | 0.1 |
| GLUC3_mmol.L | Active.B1271 / Active.B1261 | STOP | GDM | 0.985 (0.968, 1.003) | 0.10 |
67.5.4.2 Between Groups
Below are comparisons of the differences in the change in Active B12 concentration (pM) between groups. I.e. whether the change in Active B12 concentration (pM) are different between groups.
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.962618 | 0.0313047 | 1074.736 | 0.9031115 | 1.026045 | 0.242 | ns |
| STOP/SCOPE (non-GDM) | 0.981131 | 0.0159534 | 1074.736 | 0.9503218 | 1.012939 | 0.242 | ns |
| STOP/SCOPE (GDM) | 0.981131 | 0.0159534 | 1074.736 | 0.9503218 | 1.012939 | 0.242 | ns |
| GDM/non-GDM (SCOPE) | 1.002941 | 0.0244402 | 1057.071 | 0.9561131 | 1.052063 | 0.904 | ns |
| GDM/non-GDM (STOP) | 1.002941 | 0.0244402 | 1057.071 | 0.9561131 | 1.052063 | 0.904 | ns |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.006290 | 0.0175121 | 1052.3229 | 0.9725073 | 1.041246 | 0.719 | ns |
| STOP/SCOPE (non-GDM) | 1.003140 | 0.0087287 | 1052.3229 | 0.9861578 | 1.020415 | 0.719 | ns |
| STOP/SCOPE (GDM) | 1.003140 | 0.0087287 | 1052.3229 | 0.9861578 | 1.020415 | 0.719 | ns |
| GDM/non-GDM (SCOPE) | 1.025411 | 0.0131357 | 958.8608 | 0.9999541 | 1.051515 | 0.050 | ns |
| GDM/non-GDM (STOP) | 1.025411 | 0.0131357 | 958.8608 | 0.9999541 | 1.051515 | 0.050 | ns |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.9539858 | 0.0227571 | 1107.714 | 0.9103627 | 0.9996992 | 0.049 | * |
| STOP/SCOPE (non-GDM) | 0.9767220 | 0.0116497 | 1107.714 | 0.9541293 | 0.9998496 | 0.049 | * |
| STOP/SCOPE (GDM) | 0.9767220 | 0.0116497 | 1107.714 | 0.9541293 | 0.9998496 | 0.049 | * |
| GDM/non-GDM (SCOPE) | 1.0197965 | 0.0183647 | 1081.377 | 0.9843912 | 1.0564752 | 0.277 | ns |
| GDM/non-GDM (STOP) | 1.0197965 | 0.0183647 | 1081.377 | 0.9843912 | 1.0564752 | 0.277 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0284138 | 0.0865689 | Inf | 0.8719995 | 1.212885 | 0.739 | ns |
| STOP/SCOPE (non-GDM) | 0.9673981 | 0.0237372 | Inf | 0.9219751 | 1.015059 | 0.177 | ns |
| STOP/SCOPE (GDM) | 1.0630720 | 0.0857006 | Inf | 0.9076998 | 1.245040 | 0.448 | ns |
| GDM/non-GDM (SCOPE) | 0.8892404 | 0.0647259 | Inf | 0.7710136 | 1.025596 | 0.107 | ns |
| GDM/non-GDM (STOP) | 0.9771846 | 0.0414479 | Inf | 0.8992333 | 1.061893 | 0.586 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0334534 | 0.0973941 | Inf | 0.8591568 | 1.243109 | 0.727 | ns |
| STOP/SCOPE (non-GDM) | 0.9588863 | 0.0250776 | Inf | 0.9109735 | 1.009319 | 0.108 | ns |
| STOP/SCOPE (GDM) | 1.0777643 | 0.0976936 | Inf | 0.9023331 | 1.287303 | 0.409 | ns |
| GDM/non-GDM (SCOPE) | 0.8701767 | 0.0714202 | Inf | 0.7408744 | 1.022046 | 0.090 | ns |
| GDM/non-GDM (STOP) | 0.9780569 | 0.0454341 | Inf | 0.8929412 | 1.071286 | 0.633 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.007084 | 0.0112788 | Inf | 0.9852193 | 1.029435 | 0.528 | ns |
| STOP/SCOPE (non-GDM) | 1.003536 | 0.0056195 | Inf | 0.9925821 | 1.014611 | 0.528 | ns |
| STOP/SCOPE (GDM) | 1.003536 | 0.0056195 | Inf | 0.9925821 | 1.014611 | 0.528 | ns |
| GDM/non-GDM (SCOPE) | 0.980328 | 0.0103639 | Inf | 0.9602242 | 1.000853 | 0.060 | ns |
| GDM/non-GDM (STOP) | 0.980328 | 0.0103639 | Inf | 0.9602242 | 1.000853 | 0.060 | ns |